The purpose of the study was to investigate the role of psychological empowerment as moderator of the relationship between job insecurity and Organizational citizenship behaviour (OCB) among the Eastern Cape Department of Health Employees. The study was motivated because no previous study has combined psychological empowerment, job insecurity and OCB in a single study, and in a Provincial government department. Three instruments were used in this study for data collection namely; Psychological Empowerment Scale developed by Spreitzer (1995), Job Insecurity scale developed by De Witte (2000) and Organizational Citizenship Behaviour Check-list (OCB-C) developed by Fox and Spector (2009). Non-probability convenience sampling method was employed to select a sample of 281 employees, which includes 111 male and 170 female. The findings revealed that the employees’ job insecurity level negatively correlates with their organizational citizenship behaviour. It indicated that the employees’ psychological empowerment significantly and positively correlates with their OCB. It added that the psychological empowerment of the employees significantly moderates the relationship between their job insecurity and OCB. The study provided organizational leaders with the best empowerment approach to streamline their workers’ attitude and commitment towards work. It encourages human resource practitioners and organizational managers to develop and implement training and other intervention programme aimed at promoting and sustaining the OCBs of their employees. The study contributed to the overall body of knowledge and adds valuable literature on psychological empowerment, job insecurity and organizational citizenship behaviours within government departments’ context.