The study evaluated the effect of turnover of skilled staff on the patient flow rate of tertiary health institutions in Ebonyi State. The specific objectives were to: examine the effect of turnover of advanced education staff on patient medical care and evaluate the effect of turnover of experienced staff on the patient physical resources of tertiary health institutions in Ebonyi State. A total population for the study was four thousand one hundred and twenty five (4125). The sample size of 351 was drawn using Freund and William’s formula at 5 percent error margin. A survey design was adopted for the study. Instrument used for data collection was the questionnaire. A total of three hundred and fifty one(351) copies of questionnaire were distributed while three hundred and twenty five (325) copies of questionnaire were returned. Z-test was used to test the hypotheses. The findings indicated that Turnover of advanced education staff had positive significant effect on patient medical care of tertiary health institutions in Ebonyi State Z (95,n = 325), 6.989 < 10.026 = p. < 0.05. Turnover of experienced staff had significant positive effect on the physical resources of tertiary health institutions in Ebonyi state Z (95,n = 325), 9.028 < 10.692 = p. < 0.05. The study concluded that turnover of advanced education of staff and experienced staff had significant positive effect on patient medical care and physical resources of tertiary health institutions in Ebonyi state. The study recommended among others that the management should endeavour to reduce Staff turnover for the effectiveness of the human resources management system and the overall management of an organization.